One cannot help but to stand in awe of the rapid progress made in the medical field. New techniques and medicines are introduced at an astonishing rate. Of course, one of the main fields of research is diagnostics. No one will argue the fact that an early diagnosis ensures a better prognosis and lower treatment costs. ELISA kits, an enzyme linked immunosorbent assay, allow doctors to diagnose a variety of conditions quickly and efficiently.
The enzyme linked immunosorbent assay, or EIA as it is known for short is performed in a laboratory. The test is easy to run and many tests can be performed at the same time. The purpose of the test is to measure the concentration of both antigens and antibodies in the blood sample. The ability to measure this concentration is a very important part of the diagnostic process.
When there are antibodies present in a blood sample it means that the body has produced them to counteract the effects of a harmful substance, called antigens. If there are antibodies present, it is certain that there are also antigens because the antibodies are only produced when a threat is detected. By identifying the specific type of antibody produced by the body it is also possible to determine the exact nature of the threat.
Patients do not experience any form of discomfort during the test. A small sample of blood is removed and then sent to the laboratory. There, a technician will add the sample to a petri dish that is already prepared with antigens. When the antibodies in the blood sample bind with the specific antigen in the petri dish and identification can be made. Further enzymes are then added to see how the antibody and the antigen react to each other.
There are different variations of the EIA test but the more complex tests provide much more accurate results. Each type of test requires a different kit. The direct EIA test, for example is straightforward and results are available very quickly. In this case the antigens are coated with multiwall plates and the antibody can only react with the antigen once it has been conjugated with enzymes.
The indirect EIA test is much more sensitive. In this case a polystyrene plate is used to cover the antigen. The antibody can only detect the antigen during two well controlled stages. The advantage of this test is that both primary and secondary antibodies can be identified at the same time. This ability may eliminate the need for further tests, thus saving both time and money.
The sandwich EIA test requires the antibodies to be matched in pairs. The first one is covered with polystyrene and the role of the second antibody is to act as a detection agent. The main purpose of this test is to find out what the concentration of the analyte is. It is an extremely accurate test and can be used to diagnose latent diseases.
The EIA test is so popular with medical experts because it is versatile, quick and very accurate. The test is easy to administer and to process too. Patients benefit because their conditions can be diagnoses at an earlier stage. This makes treatment easier and it improves the prognosis.
The enzyme linked immunosorbent assay, or EIA as it is known for short is performed in a laboratory. The test is easy to run and many tests can be performed at the same time. The purpose of the test is to measure the concentration of both antigens and antibodies in the blood sample. The ability to measure this concentration is a very important part of the diagnostic process.
When there are antibodies present in a blood sample it means that the body has produced them to counteract the effects of a harmful substance, called antigens. If there are antibodies present, it is certain that there are also antigens because the antibodies are only produced when a threat is detected. By identifying the specific type of antibody produced by the body it is also possible to determine the exact nature of the threat.
Patients do not experience any form of discomfort during the test. A small sample of blood is removed and then sent to the laboratory. There, a technician will add the sample to a petri dish that is already prepared with antigens. When the antibodies in the blood sample bind with the specific antigen in the petri dish and identification can be made. Further enzymes are then added to see how the antibody and the antigen react to each other.
There are different variations of the EIA test but the more complex tests provide much more accurate results. Each type of test requires a different kit. The direct EIA test, for example is straightforward and results are available very quickly. In this case the antigens are coated with multiwall plates and the antibody can only react with the antigen once it has been conjugated with enzymes.
The indirect EIA test is much more sensitive. In this case a polystyrene plate is used to cover the antigen. The antibody can only detect the antigen during two well controlled stages. The advantage of this test is that both primary and secondary antibodies can be identified at the same time. This ability may eliminate the need for further tests, thus saving both time and money.
The sandwich EIA test requires the antibodies to be matched in pairs. The first one is covered with polystyrene and the role of the second antibody is to act as a detection agent. The main purpose of this test is to find out what the concentration of the analyte is. It is an extremely accurate test and can be used to diagnose latent diseases.
The EIA test is so popular with medical experts because it is versatile, quick and very accurate. The test is easy to administer and to process too. Patients benefit because their conditions can be diagnoses at an earlier stage. This makes treatment easier and it improves the prognosis.
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